AK36: Genetic transformation; regeneration; secondary metabolites.
Biotechnological tools are important for multiplication and genetic enhancement of the plants by adopting technique such as genetic transformations.
AK36 are the key step towards commercial production of secondary metabolites by plant biotechnology.
1) It gives better control for scale up of cell suspension cultures under defined parameters for the production of bioactive compounds;
2) Constant regulation of conditions at various stages of bioreactor operation is possible;
3) Handling of culture such as inoculation or harvest is easy and saves time;
4) Nutrient uptake is enhanced by submerged culture conditions which stimulate multiplication rate and higher yield of bioactive compounds;
Since the biosynthetic efficiency of populations varies, a high yielding variety should be selected as a starting material. The fundamental requirement in all this is a good yield of the compound, and reduced cost compared to the natural synthesis by the plants.
The recent advances and developments in plant genetics and recombinant DNA technology have helped to improve and boost research into secondary metabolite biosynthesis. Genetic transformation would be a powerful tool for enhancing the productivity of novel secondary metabolites of limited yield.